Cryoprotectant solution and method

Details Cryoprotectant solution and method Cryoprotectant solution and method

1987-10-16

1990-12-25

Rosen, Sam

Chemistry: molecular biology and microbiology

Maintaining blood or sperm in a physiologically active state...

435 1, 4352401, 62 64, A01N 102

Patent

active

049802771

ABSTRACT:
This invention relates to an improved cryoprotectant solution for viable cells, such as sperm, ova and blood cells which contains an effective amount of betaine.

REFERENCES:
Chemical Abstracts, vol. 97 (No. 13), Abstract No. 108,676s, Oikawa.
Chemical Abstracts, vol. 89, No. 1, Abstract No. 4855t, Inoue.
Chemical Abstracts, vol. 71, (1969), Abstract No. 934y, Sakai.
Chemical Abstracts, vol. 71, (1969), Abstract No. 10471j, Sakai.
Brearly et al., Optimal Concentrations for non-penetrating Cryopreservatives of Human Blood, Sep. 14-17, 1987 (124th British Pharmaceutical Conference), Journal of Pharmaceutical Pharmacology 39 (Suppl.) 1987, p. 30.
Coughlan et al., The Role of Glycinebetaine in the Protection of Spinach Thylakoids Against Freezing Stress 1982, pp. 62-69.
Anchordoguy et al., Modes of Interaction of Cryoprotectants with Membrane phospholipids during Freezing, Jan. 15, 1987, Cryobiology pp. 324-331.
Higgins et al., A Comparative Investigation of Glycinebetaine and Dimethylsulfoxide as Liposome Cryoprotectants J. Phar. Pharmacol., 1987, 39: pp. 577-582.

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