Cryopreservation of primate embryonic stem cells

Chemistry: molecular biology and microbiology – Animal cell – per se ; composition thereof; process of... – Method of storing cells in a viable state

Reexamination Certificate

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C435S325000, C435S363000, C435S366000

Reexamination Certificate

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07811819

ABSTRACT:
Primate embryonic stem cells are cryopreserved by resuspension in a freezing medium and slow cooling at a controlled rate. In some embodiments, prior to the controlled freezing step, the suspension of cells is cooled to a temperature just below freezing, and ice crystal formation is induced. The cryopreserved cell aggregates are useful in transplantation, for experimental evaluation, and as a source of lineage and cell specific products, and as targets for the discovery of factors or molecules that can affect them.

REFERENCES:
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Fujioka et al., A Simple and Efficient Cryopreservation Method for Primate Embryonic Stem Cells, 2004, Int. J. Dev. Biol., 48: 1149-1154.
Heng et al., The Cryopreservation of Human Embryonic Stem Cells, 2004, Biotechnol. Appl. Biochem., 1-21 (manuscript).
Ji et al., Cryopreservation of Adherent Human Embryonic Stem Cells, 2004, Biotechnol. Bioeng. 88(3): 299-312.
Kim et al., Effects of Type IV Collagen and Laminin on the Cryopreservation of Human Embryonic Stem Cells, 2004, Stem Cells, 22:950-961.
Leibo et al., 1978, (Methods in Mammalian Reproduction) Methods for the Preservation of Mammalian Embryos by Freezing, Chap. 8, 179-201.
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Reubinoff et al., 2001, Effective Cryopreservation of Human Embryonic Stem Cells by the Open Pulled Straw Vitrification Method, Human Reprod., 16(10): 2187-2194.

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