Chemistry: molecular biology and microbiology – Animal cell – per se ; composition thereof; process of... – Method of storing cells in a viable state
Reexamination Certificate
2005-05-31
2010-10-12
Chen, Shin-Lin (Department: 1632)
Chemistry: molecular biology and microbiology
Animal cell, per se ; composition thereof; process of...
Method of storing cells in a viable state
C435S325000, C435S363000, C435S366000
Reexamination Certificate
active
07811819
ABSTRACT:
Primate embryonic stem cells are cryopreserved by resuspension in a freezing medium and slow cooling at a controlled rate. In some embodiments, prior to the controlled freezing step, the suspension of cells is cooled to a temperature just below freezing, and ice crystal formation is induced. The cryopreserved cell aggregates are useful in transplantation, for experimental evaluation, and as a source of lineage and cell specific products, and as targets for the discovery of factors or molecules that can affect them.
REFERENCES:
Heng et al., Apr. 2005, Biotechnology and Applied Biochemistry, vol. 41, Part 2, pp. 97-104.
Ware et al., Jun. 2005, BioTechniques, vol. 38, No. 6, pp. 879-880, 882-883.
Richards, et al., 2004, Stem Cells, vol. 22, p. 779-789.
Ha et al., 2005, Human Reproduction, vol. 20, No. 7, pp. 1779-1785.
Fujioka et al., A Simple and Efficient Cryopreservation Method for Primate Embryonic Stem Cells, 2004, Int. J. Dev. Biol., 48: 1149-1154.
Heng et al., The Cryopreservation of Human Embryonic Stem Cells, 2004, Biotechnol. Appl. Biochem., 1-21 (manuscript).
Ji et al., Cryopreservation of Adherent Human Embryonic Stem Cells, 2004, Biotechnol. Bioeng. 88(3): 299-312.
Kim et al., Effects of Type IV Collagen and Laminin on the Cryopreservation of Human Embryonic Stem Cells, 2004, Stem Cells, 22:950-961.
Leibo et al., 1978, (Methods in Mammalian Reproduction) Methods for the Preservation of Mammalian Embryos by Freezing, Chap. 8, 179-201.
Leibo et al., 1981, (New Technologies in Animal Breeding) Preservation of Ova and Embryos by Freezing, Academic Press, Inc., 1981, 127-139.
Mazur et al., (The Freezing of Mammalian Embryos) Slow Freezing Injury in Mammalian Cells, 1977, 19-48.
Reubinoff et al., 2001, Effective Cryopreservation of Human Embryonic Stem Cells by the Open Pulled Straw Vitrification Method, Human Reprod., 16(10): 2187-2194.
Nelson Angelique M.
Ware Carol
Bozicevic Field & Francis LLP
Chen Shin-Lin
Sherwood Pamela J.
The University of Washington
LandOfFree
Cryopreservation of primate embryonic stem cells does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Cryopreservation of primate embryonic stem cells, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Cryopreservation of primate embryonic stem cells will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-4183343