Chemistry: molecular biology and microbiology – Animal cell – per se ; composition thereof; process of... – Method of storing cells in a viable state
Patent
1996-11-19
1998-10-27
Saunders, David
Chemistry: molecular biology and microbiology
Animal cell, per se ; composition thereof; process of...
Method of storing cells in a viable state
435 13, 435 2, 435372, 435375, 424 937, 424 9372, A01N 102, C12N 502
Patent
active
058277412
ABSTRACT:
The combination of trehalose and dimethyl sulfoxide is an unusually effective cryoprotectant for islets and islet-like cell clusters, as well as platelets. Islets and ICCs, when cooled through the thermotropic phase transition in the presence of this combination of treatment agents and then returned to physiologic temperature, retain their functionality. Likewise, platelets can be similarly cooled and warmed without undergoing premature activation. In general, trehalose can be incorporated into eukaryotic cells in general by suspending the cells in a trehalose solution and either cooling or warming the solution through the thermotropic transition of the cells.
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Beattie Gillian M.
Crowe John H.
Hayek Alberto
Tablin Fern
Saunders David
The Regents of the University of California
VanderVegt F. Pierre
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