Chemistry: molecular biology and microbiology – Plant cell or cell line – per se ; composition thereof;... – Culture – maintenance – or preservation techniques – per se
Patent
1997-05-20
2000-11-07
Naff, David M.
Chemistry: molecular biology and microbiology
Plant cell or cell line, per se ; composition thereof;...
Culture, maintenance, or preservation techniques, per se
435410, 435412, 435420, 435424, 435431, C12N 500, C12N 502, C12N 504
Patent
active
061435635
ABSTRACT:
A method for cryopreserving plant callus without the use of cryoprotectants or programmable freezers. The callus is spread on a physical support, grown on a media supplemented with an osmoticum, desiccated under controlled conditions, placed directly into cold storage, and can be revived by thawing. Actively growing callus from a number of species can be cryopreserved using the instant invention.
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Naff David M.
Pioneer Hi-Bred International , Inc.
Ware Deborah K.
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