Cryopreservation of cultured epithelial sheets

Details Cryopreservation of cultured epithelial sheets Cryopreservation of cultured epithelial sheets

1990-06-04

1992-09-08

Doll, John

Chemistry: molecular biology and microbiology

Differentiated tissue or organ other than blood, per se, or...

Including perfusion; composition therefor

4352401, 424574, 62 62, C12N 500, A61K 3536, A01N 102

Patent

active

051457709

ABSTRACT:
Disclosed is a method of cryopreserving a sheet of living, cultured epithelial cells for use as a skin wound dressing. The method ensures that the integrity of the sheet will be maintained, and it preserves a significant number of cells in a mitotically competent, physiologically healthy form. It involves use of a non cell penetrating cryoprotectant and certain freezing and thawing conditions.

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