Cryopreservation of biological materials in a non-frozen or vitr

Details Cryopreservation of biological materials in a non-frozen or vitr Cryopreservation of biological materials in a non-frozen or vitr

1982-11-23

1985-12-17

Rosen, Sam

Chemistry: molecular biology and microbiology

Differentiated tissue or organ other than blood, per se, or...

Including perfusion; composition therefor

62 67, 62 78, 435240, 435241, A01N 102, F25C 100

Patent

active

045592985

ABSTRACT:
A method is provided for the successful cryopreservation of biological materials including whole organs, organ sections, tissues and cells, in a non-frozen (vitreous) state, comprising cooling the biological material to be preserved under pressure in the presence of a non-toxic vitrifable protective solution to at least the glass transition temperature thereof to vitrify the solution without substantial nucleation or ice crystal growth and without significant injury to the biomaterial. The invention also provides non-toxic protective vitrification solutions useful in the cryopreservation of biomaterials.

REFERENCES:
patent: 3677024 (1972-07-01), Segall
patent: 3940943 (1976-03-01), Sikes
MacFarlane et al.--Cryo-Letters 2, 353-358 (1981).
Fahy et al.--Cryobiology, vol. 21 (1984) pp. 407-426.
Cryonics, Issue No. 12, Jul. 1981.
Abstract: Fahy, VII International Biophysics Congress and PanAmerican Biochemistry Congress, Mexico City, Aug. 23-28, 1981.
Abstract: Fahy, The Transplantation Society, Cambridge, England Apr. 6-9, 1981.
Abstract: Fahy, Society for Cryobiology, St. Louis, Missouri Jun. 14-18, 1981.
Note: Cryobiology 18: 617, 1981 (Fahy).

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