Cryogenic suspension method

Chemistry: molecular biology and microbiology – Differentiated tissue or organ other than blood – per se – or... – Including perfusion; composition therefor

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435 2, A01N 102

Patent

active

050843770

ABSTRACT:
A unique and novel operative means and method has been designed to successfully procure and sustain biological systems placed in a state of cryogenic suspension. Entire biological systems or their constituent parts are placed in a non-frozen vitrifiable state for a time interval of determinant length in the presence of a non-toxic, protective profusiate, under sustained pressure and temperature approaching or less than the glass transition temperature. The implementation of a substantial regimen of cryoprotectants and baroprotectives within the contexts of a protective profusion solution favorably alters the colligative properties of the said biological systems significantly impeding the process of nucleation. The deleterious process of nucleation, the subsequent formation of crystals and or the critical alignment of said crystals is prevented by the exactation of specific resonate energy fields. The viability of said biological systems are further maintained upon their recovery from cryogenic suspension by a series of unique and novel base profusiates, metabolic stablants and the subsequent application of thermal kinetic parameters to the aforementioned biologicals by thermal induction, microwave lasers and radiofrequency generators.

REFERENCES:
patent: 4462215 (1984-07-01), Kuraoka et al.

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