Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Stablizing an enzyme by forming a mixture – an adduct or a...
Patent
1986-10-30
1990-01-30
Wiseman, Thomas G.
Chemistry: molecular biology and microbiology
Enzyme , proenzyme; compositions thereof; process for...
Stablizing an enzyme by forming a mixture, an adduct or a...
435194, 435814, C12N 996, C12N 912
Patent
active
048973530
ABSTRACT:
A method of protecting soluble proteins such that their biological activity is preserved after freezing by exposing the protein to an amino acid or trimethylamine-N-oxide and transition metal ion prior to freezing. The protected protein can then be thawed without denaturation or impairment of the protein's biological activity. The protein is preferably exposed to the amino acid or trimethylamine-N-oxide by placing it in a 25-100 mM aqueous solution of organic solute and 1 mM Zn.sup.+2. This method is especially effective in preserving the biological activity of fragile proteins such as the enzyme phosphofructokinase. The present method can be used to preserve pharmaceutically useful proteins in a frozen form for storage and distribution. The treated protein can be thawed and administered directly to a user without removing the cryoprotectant since the amino acid or oxide and trace amounts of many transition metal ions are nontoxic.
REFERENCES:
patent: 3124517 (1964-03-01), Eloy
patent: 4169012 (1979-09-01), Dawson et al.
Carpenter, J. F. et al. (1986), Arch. Biochem. Biophys. 250(2), 505-512.
Hand, S. C. et al. (1982), J. Biol. Chem., 257(2), 734-741.
Heber, U. et al. (1971), Biochim. Biophys. Acta 241, 578-592.
Carpenter John F.
Crowe John H.
Crowe Lois M.
Hand Steven C.
Patterson Jr. Charles L.
University of Southwestern Louisiana
Wiseman Thomas G.
LandOfFree
Cryogenic protection of phosphofructokinase using amino acids an does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Cryogenic protection of phosphofructokinase using amino acids an, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Cryogenic protection of phosphofructokinase using amino acids an will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-1923271