Drug – bio-affecting and body treating compositions – Preparations characterized by special physical form – Liposomes
Patent
1986-03-13
1989-02-21
Lovering, Richard D.
Drug, bio-affecting and body treating compositions
Preparations characterized by special physical form
Liposomes
34 5, 264 43, 424 943, 4284022, 435188, 436829, 514 1, 514 3, 514 6, 514779, 514971, A61K 3722, A61K 964, A61K 3140, B01J 1302
Patent
active
048063436
ABSTRACT:
A method of protecting soluble proteins such that their biological activity is preserved after freezing by exposing the protein to a carbohydrate and transition metal ion prior to freezing. The protected protein can then be thawed or lyophilized and rehydrated without denaturation of impairment of the protein's biological activity. The protein is preferably exposed to the carbohydrate by placing it in a 25-100 mM aqueous solution of carbohydrate and 2 mM Z.sup.+2. This method is especially effective in preserving the biological activity of fragile proteins such as the enzyme phosphofructokinase. The present method can be used to preserve pharmaceutically useful proteins in a frozen or freeze-dried form for storage and distribution. The treated protein can be thawed or rehydrated and administered directly to a user without removing the cryoprotectant since the carbohydrates and trace amounts of many transition metal ions are nontoxic.
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Carpenter John F.
Crowe John H.
Crowe Lois M.
Hand Steven C.
Lovering Richard D.
University of Southwestern Louisiana
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