Chemistry: analytical and immunological testing – Involving an insoluble carrier for immobilizing immunochemicals
Patent
1993-08-03
1996-06-11
Housel, James C.
Chemistry: analytical and immunological testing
Involving an insoluble carrier for immobilizing immunochemicals
435 79, 435962, 436538, 436816, 436817, 436822, G01N 33543, G01N 3391, G01N 3374
Patent
active
055255248
ABSTRACT:
Assay for detecting the amount or presence of target ligand in a sample. The assay includes a ligand analogue conjugate having a linkage site and a binding site, a ligand receptor, and a sample. The assay includes the steps of providing at least one crosstalk inhibitor. This inhibitor, under assay conditions, competes with the linkage site of the ligand analogue conjugate for binding to the ligand receptor, and does not compete with the binding site of the ligand analogue conjugate for binding to the ligand receptor. In the invention, the assay is performed for the target ligand in the presence of a sufficient amount of the crosstalk inhibitor to reduce the amount of binding of the linkage site of the ligand analogue conjugate to the ligand receptor. The invention also features a method for identifying crosstalk inhibitors, and the crosstalk inhibitors themselves.
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Anderson Richard R.
Buechler Kenneth F.
Lee Theodore T.
Valkirs Gunars E.
Biosite Diagnostics, Inc.
Housel James C.
Parsons Nancy J.
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