Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...
Patent
1986-11-18
1989-11-07
Richman, Barry S.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving antigen-antibody binding, specific binding protein...
436525, 436526, 436501, 424 3, 424 71, 435 6, G01N 3353
Patent
active
048792209
ABSTRACT:
A method and probes are disclosed for the localization of functional receptors by electron microscopy (EM). Electron dense probes are directed to receptors by means of specific ligand-receptor interactions and subsequently anchored covalently to target cellular material. A particular probe is a complex between colloidal gold and dextran derivatized with diaminohexane and containing photo-activatable nitroarylazido groups. Ligands with a specific affinity for certain receptors can be covalently attached to the complex to provide receptor-specific markers. For example, ouabain can be covalently attached to the dextran matrix to produce a probe specific for functional units of Na,K-ATPase. Covalent attachment of the bound probes at or near their respective receptors greatly enhances retention of probe during washing and standard procedures of fixation and dehydration in preparation for EM.
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Birrell G. Bruce
Mrsny Randall J.
Volwerk Johannes J.
Alfandary-Alexander Lyle
Richman Barry S.
State of Oregon Acting By and Through the State Board of Higher
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