Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Patent
1995-06-07
1998-12-15
LeGuyader, John L.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
536 231, 536 243, C12Q 168, C07H 2104
Patent
active
058494820
ABSTRACT:
Oligonucleotides (ODNs) include a sequence that is complementary to a target sequence in single stranded RNA, or single or double stranded DNA, and an alkylating function which after hybridization alkylates the target sequence. ODNs adapted for alkylating single stranded RNA, such as messenger RNA, are complementary to the target sequence in the Watson Crick sense. ODNs adapted for alkylating double stranded DNA have at least two alkylating functions and are complementary to the target sequence in the Hoogsteen or reverse Hoogsteen sense. With these ODNs both strands of the target sequence are alkylated. A third class of ODNs have at least approximately 26 nucleotide units in a continous sequence which are complementary to the target sequence of double stranded DNA, and the alkylating function is covalently attached to a nucleotide unit in the continuous sequence. Alkylation or cross-linking with this class of ODNs occurs in the presence of a recombinase enzyme.
REFERENCES:
H. Moser et al Science 238 (Oct. 30, 1987) 645-650.
J. Hartley et al. Biochem. 29 ('90) 2985-91.
V. Vlassov et al. Gene 72 ('88) 313-22.
E. Uhlmann et al. Chem. Rev. 90(4) ('90) 543-84.
J. Milligan et al. J. Med. Chem. 36(14) ('93) 1926-37.
C. Srein et al. Science 261 (Aug. 1993) 1004-12.
B. Tseng et al Cancer Gene Ther. 1(1) (94) 65-71.
T. Brown et al., in Oligontides and Analogues, A. Practical Approach, Ed. F. Eckstein, IRL Press, Oxford U.K., 1991, pp. 1-24.
L. Pauling Chem. & Eng. News 24(10):1375-7 ('46).
R. Dickerson et al. The Stnc. & Action of Protis. Benjamin/Cumming Publ. Co., Menlo Park, CA, 1969, p. 68.
Gall Alexander A.
Gamper Howard B.
Hurst Gerald D.
Kutyavin Igor V.
Meyer, Jr. Rich B.
Epoch Pharmaceuticals, Inc.
LeGuyader John L.
LandOfFree
Crosslinking oligonucleotides does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Crosslinking oligonucleotides, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Crosslinking oligonucleotides will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-1456461