Crosslinked polyacrylamide-sulfhydryl polymer for immobilization

Chemistry: molecular biology and microbiology – Carrier-bound or immobilized enzyme or microbial cell;... – Enzyme or microbial cell is immobilized on or in an organic...

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435181, C12N 1108, C12N 1106

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active

048988243

ABSTRACT:
A water-insoluble, crosslinked polyacrylamide copolymer is prepared having recurring monomeric residues of acrylamide, bisacrylamide or analogs thereof, and N,N'-bisacrylylcystamine or analogs thereof. The crosslinked copolymer can be formed into discrete particles having crosslinking disulfide groups on their external surface which can be reduced to provide activated particles having exposed chemically active sulfhydryl groups. The activated polyacrylamide particles are free of residual amino and carboxylic acid groups and can be reacted with suitable haptens and other biologically active substances to provide insolubilized forms thereof.

REFERENCES:
patent: 3666733 (1972-05-01), Epton
patent: 3775253 (1973-11-01), Dieter et al.
patent: 4175073 (1979-11-01), Carlsson et al.
patent: 4737258 (1988-04-01), Ogawa et al.
Inman, J. K., Methods In Enzymology, vol. XXXIV, part B, Academic Press, N.Y., 1974, pp. 30-33, 42-45.

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