Synthetic resins or natural rubbers -- part of the class 520 ser – Synthetic resins – Mixing of two or more solid polymers; mixing of solid...
Patent
1991-11-08
1993-10-19
Schofer, Joseph L.
Synthetic resins or natural rubbers -- part of the class 520 ser
Synthetic resins
Mixing of two or more solid polymers; mixing of solid...
5253297, 5253301, 525355, 5253591, 5253593, 5253595, 5253596, 525383, 525379, 526320, 526321, 526325, 526332, C08F 2020
Patent
active
052546349
DESCRIPTION:
BRIEF SUMMARY
TECHNICAL FIELD
This invention relates to hydrophilic crosslinked copolymer particles obtained from glycerol monomethacrylate as a monomer, hydrophobic crosslinked copolymer particles comprising said hydrophilic crosslinked copolymer particles, as a basic structure, and having adsorption active sites in the side chain thereof, and processes for producing these polymer particles. Specifically, it relates to hydrophobic crosslinked copolymer particles and hydrophilic crosslinked copolymer particles suitable for separation of water-soluble biopolymers such as proteins, and processes for producing the same.
TECHNICAL BACKGROUND
Important requirements for carriers for adsorption separation of water-soluble biopolymers such as proteins (hereinafter referred to as "proteins, etc.") are to have such a chemical structure for the carrier to exhibit sufficient hydrophilic properties, active sites for causing a hydrophobic interaction with proteins, etc., and a proper skeleton structure and a proper specific surface area for efficient contact with proteins, etc.
A crosslinked and insolubilized polysaccharide, e.g., agarose, as a carrier with an alkyl group being introduced is known as such an adsorbent. Having a saccharide chain skeleton, such an adsorbent, however, has insufficient physical strength for industrial use. An adsorbent comprising a porous body, e.g., silica gel, having introduced thereinto a hydrophobic functional group has been proposed, but it is also unsuitable for industrial use due to lack of chemical stability against acid or alkali.
A polymer obtained by reacting glycidyl methacrylate with a crosslinking agent, hydrolyzing the glycidyl group of the resulting crosslinked copolymer, and then reacting the copolymer with a fatty acid chloride is known for use as a carrier for reverse phase partition chromatography as disclosed in JP-A-2-36351 (the term "JP-A" as used herein means an "unexamined published Japanese patent application"). Further, a spherical porous hydrophilic crosslinked polymer obtained by suspension polymerization of a hydrophilic methacrylic ester having at least one ether linkage per molecule with a crosslinking agent is known useful as a carrier for gel chromatography as disclosed in JP-A-60-55009.
However, in the process wherein glycidyl methacrylate is polymerized as a monomer, followed by hydrolysis of the glycidyl group in the presence of a mineral acid, etc. as a catalyst, hydrolysis takes place on not only the glycidyl group but the ester moiety to produce a carboxyl group so that the resulting polymer fails to have sufficient performance properties as a chromatographic adsorbent for proteins, etc. In using a hydrophilic methacrylic ester having the specific structure, there is a problem in that specific polymerization conditions must be selected.
DISCLOSURE OF THE INVENTION
An object of the present invention is to provide novel crosslinked copolymer particles which have hydrophilic properties and moderate hydrophobic properties as well and are therefore useful as a carrier for adsorption separation of proteins, etc. and a process for producing the same.
The crosslinked copolymer particles according to the present invention comprise hydrophilic crosslinked copolymer particles obtained from glycerol monomethacrylate having extremely high hydrophilic properties as a main component and a crosslinking polyvinyl compound, which has a hydrophobic functional group having 4 or more carbon atoms being mainly bonded to the glycerol monomethacrylate via an oxygen atom derived from a hydroxyl group located on said glycerol monomethacrylate.
The hydrophilic crosslinked copolymer particles comprising glycerol monomethacrylate are produced by suspension polymerization of glycerol monomethacrylate and a crosslinking polyvinyl compound in the presence of at least one organic solvent selected from the following (A) and (B).
(A) A monohydric alcohol having from 5 to 14 carbon atoms.
(B) A mono- or multi-valent ester having a solubility parameter of from 8 to 11 (cal/cm.sup.3).sup.1/2.
Th
REFERENCES:
patent: 4101461 (1978-07-01), Strop
patent: 4246362 (1981-01-01), Sasaki
patent: 4256843 (1981-03-01), Sasaki
C. R. Noller, "Chemistry of Organic Compounds" 3d Ed, 159, 596-597, (1965) Saunders (Philadelphia).
Honda Go
Ito Tsuyoshi
Takayanagi Hiroaki
Mitsubishi Kasei Corporation
Schofer Joseph L.
Zitomer Fred
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