Crossflow microfiltration process for the separation of biotechn

Details Crossflow microfiltration process for the separation of biotechn Crossflow microfiltration process for the separation of biotechn

1986-05-01

1988-06-14

Castel, Benoit

Liquid purification or separation

Processes

Liquid/liquid solvent or colloidal extraction or diffusing...

210641, 210651, 210653, 435212, 435219, 435222, 435814, B01D 1300, C12N 948, C12N 950

Patent

active

047510030

ABSTRACT:
The invention relates to a process for the separation of biotechnologically produced valuable materials form a cell suspension by crossflow microfiltration to obtain an high specific permeate flux while retention stays near 0% for long periods. To enable crossflow microfiltration to be carried out on an industrial scale in biotechnology in the separation of biotechnologically produced extracellular valuable materials from a cell suspension, particularly in the separation of alkaline protease for recovering enzyme, under economically acceptacle conditions, alkaline protease of relatively high molecular weight, more especially enzyme >20,000 daltons, is separated from a fermenter solution using polysulfone tubes having micropore diameters of from 0.3 to 0.5 .mu.m at a rate of flow of the fermenter solution of from 3 to 6 m/s parallel to the membrane surface and with a pressure difference between the concentrate side and the permeate side of 2 bar, the ratio of the mean pore diameter of the membrane to the size of the microorganisms remaining in the concentrate being between 0.15 and 0.85

REFERENCES:
patent: 4191182 (1980-03-01), Popovich et al.
patent: 4212742 (1980-07-01), Solomon et al.
patent: 4420398 (1983-12-01), Castino
Rautenbach, Robert, and Rainer Albrecht, Membrantrennver-Faitren Ultrafiltration and Umkemrosmose, 1981, Otto Salle Verlag EmpH and Co., Frankfurt, 5 pages.
Dytnerskij, Vonju. I., Membranprozesse Zur Trennung Flussiger Gemische, 1977, pp. 5, 18, 19, 22, 23, 120, 121, 129, Veb Deutscher Verlas, Leipzig.
Schweitzer, Philip A., Editor, Handbook of Separation Techniques for Chemical Engineers, 1979, pp. 2-80, 2-84 and 2-85, McGraw-Hill Book Co., N.Y.
Kroner, K. H. et al., "Problems and Improvements of Cross-Flow-Filtration in Enzyme Recovery Processes", Third Eurp. Cong. on Biotechnology, Munich 1984, vol. 3, pp. III--549 to 555.
"Development Studies of Crossflow Microfiltrations," R. Bertera, H. Steven, M. Metcalfe, The Chemical Engineer, Jun. 1984, pp. 10, 11, 13 and 14.
"Mikrofiltration Von Fermenterbruhen" Chem. Ing. Tech., 57, (1985), No. 9, pp. 747-753; Raehse and Carduck.
"Tangential Flow Filtration-A New Method for the Separation of Bacterial Enzymes From Cell Debris" (1983), vol. 5, No. 4, pp. 277-282 in Biotechnology Letters.

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