Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing compound containing saccharide radical
Patent
1991-10-01
1995-05-16
Schwartz, Richard A.
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Preparing compound containing saccharide radical
435 711, 435 712, 4351723, 43525233, 43525421, C12N 1500, C12N 119, C12N 121, C12N 1509
Patent
active
054160081
ABSTRACT:
Recombinant cells providing for the controlled expression of product proteins by way of cross-regulation between interacting operons. A structural gene for a product protein and a structural gene for a repressor of a second operon are included in a first operon. A protein encoded by a structural gene of the second operon is a repressor of the first operon. The second operon may reside on a plasmid or a chromosome of the host cell. The present invention provides for controlled expression of product protein over a range of copy numbers, as well as high transcription efficiency in the induced state. The invention includes methods for the controlled expression of product protein by recombinant cells.
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Bailey James E.
Chen Wilfred
Kallio Pauli
California Institute of Technology
Schwartz Richard A.
Vogel Nancy T.
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