Cross-linkers with high reactivity and solubility and their...

Drug – bio-affecting and body treating compositions – Conjugate or complex of monoclonal or polyclonal antibody,...

Reexamination Certificate

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C530S391100, C540S456000, C540S462000, C546S278400, C546S278700

Reexamination Certificate

active

06913748

ABSTRACT:
Disclosed is a method of making conjugates of cell binding agents and small molecule drugs comprising reacting a cell binding agent with a bifunctional cross-linking moiety to thereby provide the cell binding agent with a reactive disulfide group and then reacting the modified cell binding agent with a small molecule drug comprising a free thiol group. Bifunctional cross-linking moieties are also disclosed.

REFERENCES:
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patent: 6436931 (2002-08-01), Chari et al.
patent: 6441162 (2002-08-01), Yasui et al.
patent: 6441163 (2002-08-01), Chari et al.
patent: 6716821 (2004-04-01), Zhao et al.
patent: 2004/0235840 (2004-11-01), Chari et al.
Yokoyama et al, Makromol. Chem, 190, 2041-2054 (1989).
Yokoyama et al. “Molecular design for missile drug: Synthesis of adriamycin conjugated with immunoglobulin G using poly(ethylene glycol-block-(aspartic acid) as intermediate carrier.” Makromol. Chem. 1989, vol. 190 pp. 2041-2054, see p. 2047, and p. 2050.

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