Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Hydrolase
Patent
1996-01-26
1999-11-02
Wax, Robert A.
Chemistry: molecular biology and microbiology
Enzyme , proenzyme; compositions thereof; process for...
Hydrolase
435188, 435 76, 530300, 5303871, 530402, G01N 148, C12N 996, C12N 938, A61K 3800
Patent
active
059768576
ABSTRACT:
Formation of an intramolecular cross-link in enzyme donor polypeptide fragments of .beta.-galactosidase, thereby forming a cyclic enzyme donor which is hindered from complementation with an enzyme acceptor fragment to form active of .beta.-galactosidase. The cyclic enzyme donor can be linearized by cleaving to restore complementation ability. Assays in which such cyclic enzyme donors are linearized by specific analytes are disclosed, as well as novel homobifunctional bis-maleimido cross-linking agents of the formula ##STR1## wherein R is hydroxy or acetate.
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Dissertation by Karsten Machholz, University of Gottingen, copyright by Cuvillier Verlag in 1995, accompanied by English translation of excerpts of the dissertation, prepared by Boehringer Mannheim Corporation, Indianapolis.
Eisenbeis Scott J.
Khanna Pyare
Lingenfelter David
Powell Michael J.
Tietze Lutz F.
Boehringer Mannheim Corporation
Saidha Tekchand
Wax Robert A.
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