Cromolyn binding protein in highly purifed form, and methods for

Details Cromolyn binding protein in highly purifed form, and methods for Cromolyn binding protein in highly purifed form, and methods for

1987-07-27

1991-02-26

Draper, Garnette

Chemistry: natural resins or derivatives; peptides or proteins;

Proteins, i.e., more than 100 amino acid residues

530413, 530395, 530380, 530830, 530802, C07K 1506, C07K 320, C07K 1514

Patent

active

049962965

ABSTRACT:
Substantially pure cromolyn binding protein is prepared by means of affinity chromatography of cromolyn derivatives bound to insoluble matrices. Aminocromolyn is prepared by a six-step synthesis and amine derivatives thereof are prepared by conventional means. Obtaining a compound having an amine group instead of the OH group at the 2-carbon of the propane link of cromolyn permits many kinds of reactions without interfering with the portion of the cromolyn molecule with causes its pharmacological activity. The cromolyn derivatives can be conjugated to proteins such as BSA by means of glutaraldehyde cross-linking and such conjugates can be covalently bound to agarose beads. Cromolyn binding protein can be isolated by passing lysates of RBL-2H3 cells through chromatographic columns packed with such beads. The cromolyn binding protein can be further purified by means of lectin-agarose columns.

REFERENCES:
patent: 4683135 (1987-07-01), Pecht et al.
Mazurek et al., EMBO J., 1(5), 585-90, (1982).
Mazurek et al., PNAS, 80, 6014-18, (1983(Oct.)).
Mazurek et al., PNAS, 81, 6841-5, (Nov. 1984).

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