Cromatography process for recovering a substance or a group...

Organic compounds -- part of the class 532-570 series – Organic compounds – Fatty compounds having an acid moiety which contains the...

Reexamination Certificate

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C554S165000, C554S175000, C554S193000, C554S194000, C554S196000, C554S205000

Reexamination Certificate

active

08063235

ABSTRACT:
Method for improving the performance of a preparative batchwise Super Critical Fluid Chromatographic separation for the recovery of a target fatty acid or derivative thereof, or of a group of target fatty acids or of derivatives thereof from a mixture, characterized in that the said Super Critical Fluid Chromatographic separation employs a chromatographic column charged with a stationary phase chosen from the group consisting of aluminium oxide or aluminium oxides, titanium oxide or titanium oxides, silica gel or silica gels, amino propyl modified silica gels, diol modified silica gels, phenyl modified silica gels and “reversed phase” phases, the former in turn chosen from the group consisting of RP18, RP8 and RP3, and in that the said Super Critical Fluid Chromatographic Separation further employs a mobile phase chosen from the group consisting of ethylene, propane, ammonia, dinitrogen dioxide and CO2, wherein in the said method, during the run of the said mixture on the chromatographic column, a pressure and/or temperature gradient is applied to the chromatographic column during the elution of the target fatty acid or derivative thereof.

REFERENCES:
patent: 0379963 (1990-08-01), None
Alkio, M. et al. 2000 “Purification of polyunsaturated fatty acid esters from tuna oil with supercritical fluid chromatography”J American Oil Chemists77:315-321.
Higashidate, S. et al. 1990 “Enrichment of eicosapentaenoic acid and docosahexaenoic acid esters from esterified fish oil by programmed extraction-elution with supercritical carbon dioxide”J Chromatography515:295-303.
Klesper, E. et al. 1988 “Gradient methods in supercritical fluid chromatography”J Supercritical Fluids1:45-69.
Mishra, V.K. et al. 1993 “Extraction and purification of ω-3 fatty acids with an emphasis on supercritical fluid extraction—A review”Food Res Int26:217-226.
Saito, S. 1995 “Research activities on supercritical fluid science and technology in Japan—A review”J Supercritical Fluids8:177-204.
Smith, R.M. 1999 “Supercritical fluids in separation science—the dreams, the reality and the future”J Chromatograpy A856:83-115.
Staby, A and Mollerup, J. 1993 “Separation of constituents of fish oil using supercritical fluids: A review of experimental solubility, extraction, and chromatographic data”Fluid Phase Equilibria91:349-386.
Nagahama, K. et al. 1993 “Separation of EPA and DHA from preconcentrated AgNO3aqueous solution by supercritical CO2, C2H4and C2H6” AlChE 1993 Annual Meeting, Nov. 12, 1993, St. Louis, MO.
Nisson, W.B. et al. 1989 “Supercritical fluid fractionation of fish oil esters using incremental pressure programming and a temperature gradient”J American Oil Chemists66:1596-1600.

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