Creatine phosphokinase determination method

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G01N 3114

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040424626

ABSTRACT:
A method of determining creatine phosphokinase activity in biological fluids comprising reacting a biological fluid sample with a coupled enzyme series in an oxygenated aqueous solution containing creatine phosphate, ADP, glucose, HK, G-6-PDH, NADP and an electron transfer agent and measuring the uptake of oxygen by the oxidation of the resulting NADPH with an oxygen-sensing electrode.

REFERENCES:
patent: 3403077 (1968-09-01), Berger et al.
patent: 3540984 (1970-11-01), Deutsch
patent: 3929580 (1975-12-01), Forgione et al.
Rosalki "An Improved Procedure for Serum Creative Phosphakinase Determination", J. Lab. & Clin. Med. 69 (1967), pp. 696-705.
Greenbaum et al "The Estimation of the Oxidized and Reduced Forms of the Nicotinamide Nucleotides" Biochem. J. 95, (1965) pp. 161-166.

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