Creatine amidinohydrolase, production thereof and use thereof

Details Creatine amidinohydrolase, production thereof and use thereof Creatine amidinohydrolase, production thereof and use thereof

2005-01-11

2005-01-11

Slobodyansky, Elizabeth (Department: 1652)

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving hydrolase

C435S227000, C435S192000, C435S252300, C435S252330, C435S320100, C536S023200

Reissue Patent

active

RE038687

ABSTRACT:
A creatine amidinohydrolase having the following physicochemical properties:Action: catalyzing the following reaction;creatine+H2O→sarcosine+ureaOptimum temperature: about 40-50° C.Optimum pH: pH about 8.0-9.0Heat stability: not more than about 50° C. (pH 7.5, 30 min)Km value for creatine in a coupling assay using a sarcosine oxidase and a peroxidase: about 3.5-10.0 mMMolecule weight: about 43,000 (SDS-PAGE)Isoelectric point: aboutdelete-start id="DEL-S-00001" date="20050111" ?3.5delete-end id="DEL-S-00001" ?insert-start id="INS-S-00001" date="20050111" ?4.5insert-end id="INS-S-00001" ?,a method for producing said enzyme, comprising culture of microorganism producing said enzyme, a method for the determination of creatine or creatinine in a sample using said enzyme, and a reagent therefor.

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