Creatine amidinohydrolase gene, a novel recombinant DNA, and a p

Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Hydrolase

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4353201, 4352523, 536 232, C12N 978, C12N 1563, C12N 121, C07H 2104

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059324660

ABSTRACT:
The present invention relates to a creatine amidinohydrolase gene coding for the amino acid sequence of SEQ ID NO. 2, a recombinant DNA comprising the creatine amidinohydrolase gene inserted into a vector DNA, and a process for producing creatine amidinohydrolase by culturing a microorganism belonging to the genus Escherichia carrying said recombinant DNA and having the ability to produce creatine amidinohydrolase in medium and then recovering creatine amidinohydrolase from the culture.
According to the present invention, creatine amidinohydrolase can be efficiently produced without adding creatine to medium.

REFERENCES:
patent: 5451520 (1995-09-01), Furukawa et al.
English abstract of Laid-Open Japanese Patent Publication 62-205786 (A).
Derwent World Patent Index Acc. No. 87-265079/38.
Derwent World Patent Index Acc. No. 95-225787/30.

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