Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Reexamination Certificate
2006-10-24
2006-10-24
Fredman, Jeffrey (Department: 1637)
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
C435S091200, C435S270000, C536S023100
Reexamination Certificate
active
07125673
ABSTRACT:
The present invention provides a method, referred to as CpG retrieval, to overcome incomplete bisulfite modification of DNA recovered from formalin-fixed tissue samples. The method involves boiling deparaffinized tissue samples in citrate buffer, followed by DNA extraction for promoter methylation analysis. In general, the extracted DNA is further modified by sodium bisulfite and then subjected to a method of promoter methylation analysis. The present invention also reports that hypermethylation of ataxia-telangiectasia-mutated gene promoter is correlated with decreased overall survival in patient with head and neck squamous cell carcinoma.
REFERENCES:
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patent: WO 01/77377 (2001-10-01), None
Ai et al. Ataxia-telangiectasia-mutated (ATM) gene in head and neck squamous cell carcinoma: promoter hypermethylation with clinical correlation in 100 cases.
Sepp et al. Rapid techniques for DNA extraction from routinely processed archival tissue for use in PCR. J Clin Pathol. Apr. 1994;47(4):318-23.
Shi et al. Antigen retrieval immunohistochemistry: past, present, and future. J Histochem Cytochem. Mar. 1997;45(3):327-43. Review.
Shiurba et al. Immunocytochemistry of formalin-fixed human brain tissues: microwave irradiation of free-floating sections. Brain Res Brain Res Protoc. Jan. 1998;2(2):109-19.
Bolt et al. The ATM/p53 pathway is commonly targeted for inactivation in squamous cell carcinoma of the head and neck(SCCHN) by multiple mechanisms. Oral Oncol. Nov. 2005;41(10):1013-20. Epub Aug. 31, 2005.
Brent Thomas
Fan Chun-Yang
Adler Benjamin Aaron
Fredman Jeffrey
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