Chemistry: electrical and wave energy – Processes and products – Electrophoresis or electro-osmosis processes and electrolyte...
Patent
1995-07-26
1998-07-28
Gorgos, Kathryn L.
Chemistry: electrical and wave energy
Processes and products
Electrophoresis or electro-osmosis processes and electrolyte...
204456, 204606, G01N 2726, G01N 27447
Patent
active
057858322
ABSTRACT:
Covalently cross-linked, mixed-bed agarose-polyacrylamide matrices for electrophoresis and chromatography are disclosed. The mixed-bed matrices are formed by the copolymerization of a substituted agarose mixture with acrylamide monomers. The disclosed matrices have improved polymerization properties enabling the formation of gels which have higher porosity and elasticity than conventional gels and which are therefore particularly suited to the separation of intermediate size DNA fragments and high molecular mass proteins.
REFERENCES:
patent: 4504641 (1985-03-01), Nochumson
P. G. Righetti et al, "Towards new formulations for polyamide matrices: N-Acryloyl amino ethoxy ethanol, a novel monomer combining high hydrophilicity with extreme hydrolytic stability" Electrophoresis 15 (1994)* 177-186.
Marcella Chiari et al, "New types of large-pore polyacrylamide-agarose mixed-bed matrices for DNA electrophoresis: Pore size estimation from Ferguson plots of DNA fragments", Electrophoresis 16 (1995)* 1337-1344.
Chiari Marcella
Righetti Pier Giorgio
Gorgos Kathryn L.
Starsiak Jr. John S.
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