Covalent attachment of nucleic acid molecules onto solid-phases

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid

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536 243, C12Q 168

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06030782&

ABSTRACT:
Methods for the covalent, specific and reversible immobilization of nucleic acid molecules onto solid-phases by means of a reversible disulfide bond for nucleic acid molecule array preparation are described. These methods can be used to prepare reusable nucleic acid molecule arrays with hilgh specificity and high efficiency.

REFERENCES:
patent: 5302509 (1994-04-01), Cheesman
patent: 5897955 (1999-04-01), Drumheller
Carlsson, J. et al., "Solid Phase Disulfide Oxides: A New Approach to Reversible Immobilization and Covalent Chromatography of Thiol Compounds," Biotech. Applied Biochem. 14:114-120 (1991).
Chu, B.C.F. et al., "Ligation of Oligonucleotides to Nucleic Acids or Proteins via Disulfide Bonds," Nucleic Acids Res. 16: 3671-3691 (1988).
Fahy, E. et al., "Design and Synthesis of Polyacrylamide-based Oligonucleotide Supports for Use in Nucleic Acid Diagnosis" Nucleic Acids Res. 21: 1819-1826 (1993).
Holmstrom, K. et al., "A Highly Sensitive and Fast Nonradioactive Method for Detection of Polymerase Chain Reaction Products," Anal Biochem. 209:278-283 (1993).
Maskos, U. et al., "Oligonucleotide Hybridisations on Glass Supports: A Novel Linker for Oligoncleotide Synthesis and Hubridisation Properties of Oligonucleotides Synthesised in situ," Nucl. Acids Res. 20:1679-1684 (1992).
Newton, C.R. et al., "The Production of PCR Products with 5' Single-Stranded Tails using Primers that Incorporated Novel Phosphoramidite Intermediates," Nucl. Acids Res. 21:1155-1162 (1993).
Rasmussen, S.R. et al., "Covalent Attachment of DNA onto Polystyrene Microwells: The Molecules are only Bound at the 5' End," Anal. Biochem. 198:138-142 (1991).
Running, J.A. et al., "A Procedure for Productive Coupling of Synthetic Oligonucleotides to Polystyrene Microtiter Wells for Hybridization Capture," BioTechniques 8:276-277 (1990).
Sliwkowski, M.X. et al., "Resolution of Sulphydryl Oxidase from gama-Glutamyltrransferase in Bovine Milk by Covalent Chromatography on Cysteinylsuccinamidopropyl-Glass," Biochem. J. 209:731-739 (1983).

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