Organic compounds -- part of the class 532-570 series – Organic compounds – Carbohydrates or derivatives
Patent
1998-02-20
2000-01-11
Marschel, Ardin H.
Organic compounds -- part of the class 532-570 series
Organic compounds
Carbohydrates or derivatives
435 6, 422 50, 422 681, C07H 2100
Patent
active
060137894
ABSTRACT:
Disclosed herein is a method for attaching pre-synthesized oligonucleotides to a polypropylene support medium. Most preferably, a polypropylene film is aminated by a plasma discharge in the presence of ammonia gas. An oligonucleotide having a terminal phosphate is activated in the presence of an imidazole and a carbodiimide to form a phosphorimidazolide. The activated oligonucleotide becomes immobilized by forming a phosphoramidate bond with the aminated polypropylene. The invention can be used to construct oligonucleotide arrays for hybridization assays.
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Beckman Coulter Inc.
Kivinski Margaret A.
Marschel Ardin H.
May William H.
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