Coupling of nucleic acids to solid support by photochemical meth

Details Coupling of nucleic acids to solid support by photochemical meth Coupling of nucleic acids to solid support by photochemical meth

1984-05-18

1987-12-15

Marantz, Sidney

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

422 57, 436 63, 436 94, 436501, 935 78, G01N 3350

Patent

active

047133260

ABSTRACT:
A solid support capable of binding a nucleic acid thereto upon suitable irradiation, comprising (a) a solid substrate, (b) a photochemically reactive intercalator compound or other nucleic acid-binding ligands, and (c) divalent radical chemically linking the substrate and the ligand (b). Specifically, a hydroxy group-containing solid substrate such as nitrocellulose paper is linked via a bifunctional reagent such as cyanogen bromide or 1,4-butanedioldiglycidyl ether to an amino-substituted angelicin or psoralen or ethidium bromide which in turn is photochemically linked to a nucleic acid. The resulting immobilized nucleic acid probe is capable of hybridizing with complementary nucleic acid fragments and is thereby useful in diagnostic assays.

REFERENCES:
patent: 4542102 (1985-09-01), Dattagupta
patent: 4582789 (1986-04-01), Sheldon
patent: 4592998 (1986-06-01), Avrameas

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