Coupling agents, the active intermediates and the conjugates...

Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Stablizing an enzyme by forming a mixture – an adduct or a...

Reexamination Certificate

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C435S006120, C435S007500, C435S007920, C546S290000, C436S544000, C436S545000, C436S546000, C530S395000, C530S409000

Reexamination Certificate

active

07132269

ABSTRACT:
The present invention concerns coupling agents having the following general formula:activated intermediates consisting of a coupling agent such as defined above with either a molecule having on its surface at least one aldehyde and/or ketone function before conjugation, or with one to eight molecules having on their surface at least one free thiol function before conjugation, conjugates consisting of a coupling agent such as defined above, of a molecule having on its surface at least one aldehyde and/or ketone function and of one to eight molecules having on their surface at least one free thiol function, and the use of said conjugates for in vitro diagnostic methods of diseases involving recognition of a ligand-anti-ligand pair.

REFERENCES:
patent: 5162505 (1992-11-01), Dean et al.
patent: 5929211 (1999-07-01), Ashkenazi et al.
patent: WO 99/13919 (1999-03-01), None
Stephen D. Mikolajczyk, et al., “High Yield, Site-Specific Coupling of N-Terminally Modified beta-Lactamase to a Proteolytically Derived Single-Sulfhydryl Murine Fab”, Bioconjugate Chem., 1994, 5, pp. 636-646.
Pierce Catalog, Pierce Chemical Company, Jan. 1999; EMCH, KMUH, MPBH, BMPH.

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