Coupled transcription and translation in eukaryotic cell-free ex

Details Coupled transcription and translation in eukaryotic cell-free ex Coupled transcription and translation in eukaryotic cell-free ex

1994-06-27

1996-02-20

Fleisher, Mindy

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Enzymatic production of a protein or polypeptide

435 691, 4353201, C12P 2100

Patent

active

054928176

ABSTRACT:
A method for coupling transcription and translation from DNA using a solution comprising a eukaryotic cell-free extract comprising adding a sufficient amount of a magnesium compound to the extract to raise the magnesium concentration to a level where RNA is transcribed from DNA and RNA translates into protein.

REFERENCES:
patent: 5324637 (1994-06-01), Thompson
Zubay, G. (1973) Ann. Rev. Genet. vol. 7, p. 267.
Pelham, H. R. B. and Jackson, R. J. (1976) Eur. J. Biochem. vol. 67, p. 247.
Walter, P. and Biobel, G. (1983) Meth. Enzymol. 96, 84.
Glass, C. A. and Pollard, K. M. (1990) Promega Notes 26.
Roberts, B. E. and Paterson, B. M. (1973) Proc. Natl. Acad. Sci. USA, vol. 70, p. 2330.
Anderson, C., et al. (1983) Meth. Enzymol. 101, 635.
Krieg, P. and Melton, D. (1984) Nucl. Acids Res., vol. 12, p. 7057.
Roberts, B. E., et al. (1975) Proc. Natl. Acad. Sci. USA, vol. 72, 1922-1926.
Pelham, H. R. B., et al. (1978), Eur. J. Biochem., vol. 82, 199-209.
Spirin, et al. (1988) Science, vol. 242, 1162-1164.
Ryabova, et al. (1989) Nucl. Acid Res., vol. 17, No. 11,4412.
Baranov, et al. (1989) Gene, vol. 84, 463-466.
Manufacturing Protocol L418, Promega Corp., Madison, Wis.
Technical Bulletin 101, Promega Corp., Madison, Wis.
in vitro Translation Technical Manual, Promega Corp., Madison, Wis.
Manufacturing Protocol L415/L416, Promega Corp., Madison, Wis.
Jackson, R. and Hunt, T. (1983) Meth. In Enzymol. 96, 50.
Suzuki (1977) J. Biochem. vol. 82, 251-260.

Affiliated with

Also associated with

Rating

Coupled transcription and translation in eukaryotic cell-free ex does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Coupled transcription and translation in eukaryotic cell-free ex, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Coupled transcription and translation in eukaryotic cell-free ex will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-1355557