Coupled transcription and translation in eukaryotic cell-free ex

Details Coupled transcription and translation in eukaryotic cell-free ex Coupled transcription and translation in eukaryotic cell-free ex

1995-02-13

1997-09-09

Jagannathan, Vasu S.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Enzymatic production of a protein or polypeptide

435 691, 4353201, C12P 2100

Patent

active

056655630

ABSTRACT:
A method for producing protein from DNA in a static reaction in which DNA, ribonucleotide triphosphates, magnesium and a RNA polymerase are mixed and incubated during which RNA is transcribed from the DNA and the resulting solution is added to a eukaryotic cell-free extract containing magnesium so that the RNA produced during the incubating step translates into protein and so that further transcription of the DNA is coupled to translation of the resulting RNA into protein.

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