Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Recombinant dna technique included in method of making a...
Patent
1994-08-23
1996-09-17
Elliott, George C.
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Recombinant dna technique included in method of making a...
536 231, C12P 2102, C07H 2104
Patent
active
055567690
ABSTRACT:
Batch and continuous-flow cell-free methods for synthesizing protein wherein translation is coupled with replication of recombinant mRNA by an RNA-directed RNA polyerase, such as Q.beta. replicase. Transcription of a DNA template to produce the recombinant mRNA can be coupled additionally with coupled transcription-replication-translation methods, both batch and continuous. The invention also includes kits of reagents for synthesizing user-selected proteins according to methods of this invention.
REFERENCES:
patent: 4786600 (1988-11-01), Kramer et al.
patent: 5324637 (1994-06-01), Thompson et al.
patent: 5478730 (1995-12-01), Alakhov et al.
Pratt et al. 1981 Mar, 9(18):4459-4474.
Spirin et al. 1988, Sci. 242:1162-1164.
Kudlicki, et al., "High Efficiency Cell-Free Synthesis of Proteins: Refinement of the Coupled Transcription/Translation System"; Analytical Biochemistry 206, 389-393 (1992).
Kolosov, et al., "Preparative in Vitro Synthesis of Bioactive Human Interleukin-2 in a Continuous Flow Translation System"; Biotechnology and Applied Biochemistry 16, 125-133 (1992).
Endo, et al., "Production of an enzymatic active protein using a continuous flow cell-free translation system"; Journal of Biotechnology, 25 (1992) 221-230.
Axelrod, et al. (1991) "Coliphage Q.beta. RNA Replication: RNA Catalytic for Single-Strand Release" Virology 184:595-608.
Baranov, et al. (1989) "Gene expression in a cell-free system on the preparative scale" Gene 84:463-466.
Baranov, et al. (1993) "Gene-Expression in Cell-Free System on Preparative Scale" Methods in Enzymology 217:123-142.
Bausch, et al. (1983) "Terminal Adenylation in the Synthesis of RNA by Q.beta. Replicase" The Journal of Biological Chemistry 258:1978-1984.
Blundell, et al. (1972) "Decay Rates of Different mRNA in E. coli and Models of Decay" Nature 238:46-49.
Domingo, et al. (1985) "The quasispecies (extremely heterogeneous) nature of viral RNA genome populations: biological relevance--a review" Gene 40:1-8.
Domingo, et al. (1978) "Nucleotide Sequence Heterogeneity of an RNA Phage Population" Cell 13:735-744.
Kamen (1975) "Structure and Function of the Q.beta. RNA Replicase" in RNA Phages (Norton Zinder ed.) pp. 203-234.
Kigawa et al. (1991) "A Continuous Cell-Free Protein Synthesis System for Coupled Transcription-Translation" J. Biochem. (Tokyo) 110:166-168.
Ko (1975) "Possible Mechanism of E. coli Messenger RNA Degradation: Non-Enzymatic Degradation" BioSystems 6:205-208.
Kolakofsky, et al. (1971) "Possible Mechanism for Transition of Viral RNA from Polysome to Replication Complex" Nature New Biology 231:42-46.
Kolakofsky, et al. (1971) "Q.beta. replicase as repressor of Q.beta. RNA-directed protein synthesis" Biochem. Biophys. Acta 246:596-599.
Kolakofsky, et al. (1973) "Resynchronization of RNA Synthesis by Coliphage Q.beta. Replicase at an Internal Site of the RNA Template" J. Mol. Biol. 76:271-284.
Kramer et al. (1978) "RNA sequencing with radioactive chain-terminating ribonucleotides" Proc. Natl. Acad. Sci. USA 75:5334-5338.
Lizardi, et al. (1988) "Exponential Amplification of Recombinant-RNA Hybridization Probes" Bio/Technology 6:1197-1202.
Lomeli, et al. (1989) "Quantitative Assays Based on the Use of Replicatable Hybridization Probes" Clin. Chem 35:1826-1831.
Lucotte, et al. (1993) "Introduction to Molecular Cloning Techniques" VCH Publishers, NY, pp. 123-132.
Melton et al. (1984) "Efficient in vitro synthesis of biologically active RNA and RNA hybridization probs from plasmids containing a bacteriophage SP6 promoter" Nucleic Acid Res. 12:7035-7056 and 7057-7070.
Miele et al., (1983) "Autocatalytic Replication of a Recombinant RNA" J. Mol. Biol. 171:281-295.
Morozov, et al. (1993) "Synergism in replication and translation of messenger RNA in a cell-free system" Proc. Natl. Acad. Sci. USA 90:9325-9329.
Munishkin et al. (1991) "Efficient Templates for Q.beta. Replicase are Formed by Recombination from Heterologous Sequences" J. Mol. Biol. 221:463-472.
Priano, et al. (1987) "Evolution of the RNA Coliphages: The Role of Secondary Structures during RNA Replication" Cold Spring Harbor Symposia on Quantitative Biology 52:321-330.
Rattan (1990) "Continuous gene expression in vitro: the Spirin system" Tibtech 8:275-276.
Ryabova, et al. (1989) "Preparative synthesis of globin in a continuous cell-free translation system from rabbit reticulocytes" Nucleic Acids Research 17:4412.
Ryabova, et al. "Continuous-Flow Cell-Free Translation, Transcription-Translation and Replication-Translation Systems" in Methods in Molecular Biology (Walker, J. N. ed) in press.
Ryabova, et al. (1994) "Coupled Replication-Translation of Amplifiable Messenger RNA" The Journal of Biological Chemistry 269:1501-1506.
Schleif, et al. (1981) "Practical Methods in Molecular Biology," Springer-Verlag, New York, pp. 56-60 and 161-166.
Spirin, et al. (1991) "Cell-Free Protein Synthesis Bioreactor" in Frontiers in Bioprocessing II (Todd, et al. eds.) American Chemical Society, Washington, D.C. pp. 31-43.
Spirin (1990) "Ribosome Preparation and Cell-Free Protein Synthesis" in The Ribosome: Structure, Function, and Evolution (Hill, W. E., et al. eds.) Amer. Soc. Microbiol., Washington, D.C. pp. 56-70.
Ugarov, et al. (1994) "Expression and stability of recombinant RQ-mRNAs in cell-free translation systems" FEBS Lett. 341:131-134.
Weber, et al. (1972) "Molecular Basis for Repressor Activity of Q.beta. Replicase" Nature 237:166-170.
Wu, et al. (1992) "Amplifiable messenger RNA" Proc. Natl. Acad. Sci USA 89:11769-11773.
Chetverin Alexander B.
Kramer Fred R.
Kurnasov Oleg V.
Morosov Igor Y.
Ryabova Lyubov A.
Brusca John S.
Elliott George C.
Hone William J.
LandOfFree
Coupled replication-translation methods and kits for protein syn does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Coupled replication-translation methods and kits for protein syn, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Coupled replication-translation methods and kits for protein syn will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-412594