Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing oxygen-containing organic compound
Patent
1996-09-06
1998-07-14
Naff, David M.
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Preparing oxygen-containing organic compound
435136, 435139, 435140, 435141, 435155, 435158, 435160, 435161, 435174, 435177, 435180, C12P 762, C12P 740, C12P 702, C12N 1100
Patent
active
057802750
ABSTRACT:
A process is provided for coupled microbial saccharide fermentation and esterification to produce an esterified fermentation product from a saccharide. A microorganism capable of fermenting a saccharide to produce a water-soluble organic acid, alcohol or acetylcoenzyme A, and capable of producing an esterase or alcohol acetyltransferase is attached to a hydrophilic carrier to immobilize the microorganism. The immobilized microorganism is contacted with a hydrophobic organic solvent having dissolved therein a water-insoluble alcohol, organic acid or aldehyde. The organic solvent at an interface is in contact with an aqueous medium containing a saccharide. The microorganism is grown at the interface to ferment the saccharide and produce a water-soluble organic acid, alcohol or acetylcoenzyme A. The esterase or alcohol acetyltransferase produced by the microorganism causes the water-soluble organic acid, alcohol or acetylcoenzyme A to undergo an esterification reaction with the water-insoluble alcohol or organic acid in the organic solvent or with a water-insoluble organic acid produced by microbial oxidation of the water-insoluble alcohol in the organic solvent or with a water-insoluble alcohol or organic acid produced by microbial oxidation of the water-insoluble aldehyde in the organic solvent to a produce an esterified fermentation product from the saccharide.
REFERENCES:
Oda, et al., Journal of Fermentation and Bioengeering, vol. 78, No. 2, 1994, pp. 149-154.
Oda, et al., Biosci. Biotech. Biochem., vol. 56, No. 12 1992, pp. 2041-2045.
Oda, et al., Bioschi. Biotech. Biochem., vol. 56, No. 9, 1992, pp. 1515-1517.
Kansai Paint Co. Ltd.
Naff David M.
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