Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing compound containing saccharide radical
Patent
1999-02-17
2000-10-10
Sisson, Bradley L.
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Preparing compound containing saccharide radical
435 6, 435 911, 435810, 536 231, 536 243, 536 2433, C12Q 168, C12P 1934, C07H 2102, C07H 2104
Patent
active
06130073&
ABSTRACT:
A method based on polymerase chain reaction (PCR) amplification and oligonucleotide ligase assay (OLA) reaction is provided for analyzing complex genetic systems in a single reaction vessel. The method involves simultaneously incubating a sample containing one or more target polynucleotides with PCR primers and OLA probes in a single reaction mixture. The presence of variant polynucleotide sequences in the sample is determined by detecting and identifying the products of the OLA reaction.
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Perkin-Elmer Corp., Applied Biosystems Division
Sisson Bradley L.
Weitz David J.
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