Coupled amplification and ligation method

Details Coupled amplification and ligation method Coupled amplification and ligation method

1996-09-19

1999-06-15

Sisson, Bradley L.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing compound containing saccharide radical

435 6, 435 911, 435810, 436 231, 436 243, 436 2433, 436 253, 935 8, 935 76, 935 77, C12P 1934, C07H 2104, C07H 2102, C12N 1500

Patent

active

059121483

ABSTRACT:
A method based on polymerase chain reaction (PCR) amplification and oligonucleotide ligase assay (OLA) reaction is provided for analyzing complex genetic systems in a single reaction vessel. The method involves simultaneously incubating a sample containing one or more target polynucleotides with PCR primers and OLA probes in a single reaction mixture. The presence of variant polynucleotide sequences in the sample is determined by detecting and identifying the products of the OLA reaction.

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