Coumermycin/novobiocin-regulated gene expression system

Details Coumermycin/novobiocin-regulated gene expression system Coumermycin/novobiocin-regulated gene expression system

2004-06-09

2010-11-23

Sajjadi, Fereydoun G (Department: 1633)

Drug, bio-affecting and body treating compositions

Designated organic active ingredient containing

Carbohydrate doai

C435S320100, C424S093200

Reexamination Certificate

active

07838501

ABSTRACT:
A chimeric transactivator comprises a transcription activation domain, a repressor protein DNA binding domain and the bacterial DNA gyrase B subunit. A target gene is operatively linked to operator DNA sequences recognized by the repressor binding domain. The addition of the antibiotic coumermycin results in a coumermycin-switched dimerization of the transactivator, which then binds to operator DNA sequences and activates transcription of the target gene. The addition of novobiocin switches off expression of the target gene by abolishing coumermycin-induced dimerization of the transactivator.

REFERENCES:
patent: 6187757 (2001-02-01), Clackson et al.
patent: 2003/0166191 (2003-09-01), Gardner et al.
Pollock et al. Nature Biotech. 20:729-733; 2002.
Mohi et al. Mol: Biol. Cell 9:3299-3308; 1998.
Mohi, M. et al. Mol. Biol. Cell 9:3299-3308; 1998.
Park, S. et al. Methods 28:346-352; 2002.
Weiss et al. Biochem. 26:897-904; 1987.
Hu et al. Structure 3:431-433; 1995.
NCBI GenBank Accession No. BAA01369; submitted Feb. 17, 1992; pp. 1-3.
Rittner et al. J. Mol. Biol. 248: 562-580; 1995.
Saez, E., No, D., West, A., and Evens, R.M. (1997) Curr. Opin. Biotehcnol. 8, 608-616.
Harvey, D., and Caskey, C. (1998) Curr. Opin Chem. 2, 512-518.
Gingrich, J. and Roder, J. (1998) Annu. Rev. Neurosci. 21, 377-405.
DeMayo, F.J., and Tsai, S.Y. (2001) Trends Endocrinol. Metab. 12, 348-353.
Urlinger, S., Baron, U., Thellmann, M., Hasan, M.T., Bujard, H., and Hillen, W. (2000) Proc. Natl. Acad. Sci U.S.A. 97, 7963-7968.
Riond, J.L., and Riviera, J.E. (1988) Vet. Human Toxicol. 5,431-443.
Farrar, M.A., Olson. S.H., and Perimutter, R.M. (2000) Methods Enzymol 327, 421-429.
Pabo, C.O., Sauer, T.T., Sturtevant, J.M., and Ptashne, M. (1979) Proc. Natl. Acad. Sci. U.S.A. 76, 1608-1612.
Weiss. M.A., Paco, C.O., Karplus, M., and Sauer. R.T. (1987) Biochemistry 26, 897-904.
Gossen, M., and Bujard, H. (1992) Proc. Natl. Acad. Sci U.S.A. 89, 5547-5551.
Kim, Y.I., and Hu, J.C. (1995) Proc. Natl. Acad. Sci. U.S.A. 92, 7510-7514.
Byrne, B.J., Davis, M.S., Yamaguchi, J., Bergsama, D.J., and Subramanian, K.N. (1983) Proc. Natl. Acad. Sci U.S.A. 80, 721-725.
Pastorino, J.G., Chen, S.-t., Tafani, M., Snyder, J.W., and Farber J.L. (1998) J. Biol. Chem. 273, 7770-7775.
Gu, J., Shang, L., Huang, X., Lin, T. Yin, M., Xu, K., Ji, L., Roth, J.A., and Fang. B. (2002) Oncogene 21, 4757-4764.
Kobayashi, T., Sawa, H., Morikawa, J., Ueno, S., Katayama, N., Zhang, W., and Shiku, H. (2002) Int. J. Oncol. 20, 723-728.
O'Farrell, A.M., Liu, Y., Moore, K.W., and Mui, A.L. (1998) EMBO J. 17, 1006-1018.
Gormley, N. A., Orphanides. G., Meyer, A., Cullis, P.M., and Maxwell, A. (1996) Biochemistry 35, 5083-5092.
Godfrey, J.C., and Price, K.E. (1972) Adv. Appl. Microbiol. 15, 231-296.
Eder, J.P., Wheeler, C.A. Teicher, B.A. and Schipper, L.E. (1991) Cancer Res. 52, 510-513.
Chrast-Balz, J., and Van Huijsduijnen, R.M. (1996) Nucleic Acids Res. 24, 2900-2904.
Amara, J.F., et al, Proceedings of the National Academy of Sciences of the United States of America, 1997, vol. 94 p. 10618-10623.
Pollock R. and Clackson T., Current Opinion in Biotechnology, 2002, vol. 13, p. 459-467.
Ali, J.A. et al, Biochemistry, 1993, vol. 32, p. 2717-2724.
Japanese Examiner's Report relating to corresponding Japanese Application No. 2006-515586, dated Mar. 17, 2010; including unofficial English translation thereof.
Adachi, I. et al, Nucleic Acids Research, 1987, vol. 15, No. 2, pp. 771-784.

Affiliated with

Also associated with

Rating

Coumermycin/novobiocin-regulated gene expression system does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Coumermycin/novobiocin-regulated gene expression system, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Coumermycin/novobiocin-regulated gene expression system will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-4187338