Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...
Patent
1988-04-04
1992-09-22
Kepplinger, Esther L.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving antigen-antibody binding, specific binding protein...
435 75, 435288, 435291, 435817, 436527, 436806, G01N 3353, G01N 33552, C12M 140
Patent
active
051496291
ABSTRACT:
The invention relates to a novel assay for the determination of entities having biological activity and to a device for carrying out such determinations. The assay is a very sensitive one and quantities in the picogram per milliliter range can be determined. The assay is based on chrono-coulometric measurements with sequential measurements of a plurality of samples being carried out with the aid of a multiplexer, in combination with a potentiostat, there being provided suitable electrodes and means for applying a predetermined voltage. One of the electrodes is advantageously a glassy carbon electrode, carbon felt or cloth or carbon paper. Amongst biologically active entities there are antibodies/antigens; hormones/receptors; nuclotides
ucleotide probes, one of the members of such a pair being immobilized on an electrode surface, being tagged with an enzyme providing a signal in coulometric measurements.
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Robinson, G. A., et al., Biological Abstracts, vol. 82, Abstract No. 91756 (1986).
Strohl, A. N., et al., Chemical Abstracts, vol. 91, No. 1, Abstract No. 2042t (1979).
Rishpon Judith
Rosen Ilara
Kepplinger Esther L.
Ramot University Authority for Applied Research and Industrial
Scheiner Toni R.
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