Cotton transformation

Chemistry: molecular biology and microbiology – Treatment of micro-organisms or enzymes with electrical or... – Modification of viruses

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4352522, 435419, 435427, 435430, 4354301, 435431, 800205, 800DIG27, 800DIG63, C12N 1582, C12N 1584, A01H 400, A01H 500

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058467971

ABSTRACT:
A method is provided for regenerating cotton plants from explant tissue. The improved method allows the generation of embryogenic callus from a cotton tissue explant which is not cultivated on cotton initiation media having exogenous plant hormones. The method can be utilized in the transformation of cotton plants, by cutting cotton tissue to form an explant, co-cultivating the cotton explant tissue with Agrobacterium comprising a DNA sequence of interest, and culturing the co-cultivated explant on cotton initiation media comprising a selective agent but having no exogenous plant hormones. In this fashion transformed cells are induced to produce embryogenic callus on hormone-free selective media.

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