Drug – bio-affecting and body treating compositions – Designated organic active ingredient containing – Carbohydrate doai
Reexamination Certificate
2002-02-01
2004-03-16
Barts, Samuel (Department: 1623)
Drug, bio-affecting and body treating compositions
Designated organic active ingredient containing
Carbohydrate doai
C514S887000, C536S004100, C536S123100
Reexamination Certificate
active
06706696
ABSTRACT:
FIELD OF THE INVENTION
The invention is situated in the area of cosmetics and concerns preparations containing mixtures of specific glucans and nucleic acids.
PRIOR ART
The pigment coating of normal skin leads under the influence of sun radiation to the formation of melanines. In this connection the irradiation with UV-A light with long wavelength leads to darkening of the melanine bodies which are already in the epidermis, without visible harmful results, whereas the UV-B radiation with short wavelengths leads to formation of new melanines. However, prior to the formation of the protective pigment, the skin is exposed to the influence of the unfiltered radiation, which, depending of exposure time, leads to red skin (erythemas), inflammation of the skin (sunburn) or also to blisters of bum. The stress on the organism in connection with such skin lesions, e.g. in connection with the distribution of histamines, can in addition lead to headache, faintness, fever, disturbance of heart and circulation and such like. For the consumer who wants to protect himself against the harmful aspects of radiation from the sun, various products are offered on the market, in the form of preparations in water and also cremes, oils and milky emulsions, which in addition to some skin care substances mainly contain UV light protection filters. However, as to the UV protection as well as the support of the regulation of the water and metabolism in the epidermis, these preparations are by no means satisfactory.
Therefore there is in still a demand in the market for products with an improved spectrum of efficiency. For the evaluation of the efficiency of such products it is above all relevant, to what extent they are capable of preventing the harmful effects of the UV radiation to the skin and hair, especially, however, to the (desoxy)ribonucleic acids of the skin. Further skin compatibility as well as the use of natural products, are in demand by the customer.
In this connection reference is made to the European patent EP-B1 0500718, as well as patents U.S. Pat. Nos. 4,891,220; 5,223,491; 5,397,773; 5,519,009; 5,576,015, 5,702,719 and 5,705,184 (Donzis), from which the use of glucans in skin care and sun protecting agents is known. The glucans are obtained through extraction from the cell walls of yeasts, they are insoluble in water and contain (1,6) linkages. Further in EP-A1 0681830 (Unilever) sun protection agents are suggested which contain ethylene/vinyl acetate copolymers and polyacrylates, UV light protection filters and optionally up to 10% by weight of glucans. Eventually, from U.S. Pat. No. 5,158,772 (Davis) compositions for the topical use on the skin are known, which among others contain an effective amount of a specific 1,3 glucan, which is obtained from
Cellulomonas flavigena.
Further, reference is made to the French patent application FR-A1 2511253, in which skin and sun protection agents with a content of DNA and with a high degree of polymerisation are suggested. From the Japanese laid open publication JP-A2 621096404 (Kanebo) cosmetic preparations with nucleic acids and diisopropylamin dichloro acetate are known. The object of the French patent FR-B1 2620024 (Soc. d'Etudes Dermatologiques) are preparations containing nucleic acid derivatives as radical capturers. Examples are adenine, guanosin, xanthin, hypoxanthin, uracil and ribonucleic acid. In the international patent application WO 95/01773 (Boston University) a method for stimulating the pigment production is described, wherein DNA fragments are transported in liposomal form in the epidermis. Finally, the object of the German patent application DE-A1 4323615 is compositions with a content of nucleic acids and their fragments as anti wrinkling creme and sun protection creme.
Consequently, the complex task of the present invention was to make available cosmetic, respectively pharmaceutical, preparations, which by topical use penetrate the upper layers of the skin, or by hair care penetrate the keratin fibers, where they protect the tissue as well as the cells in a direct manner against the harmful effects of UV radiation, and support the film formation on the hair and the regulation of the water and the metabolic functions in the skin, and in this way are causing a vitalisation.
DESCRIPTION OF THE INVENTION
The object of the invention is cosmetic and/or pharmaceutical preparations which contain
(a) water soluble &bgr;-(1,3) glucans, substantially free from &bgr;-(1,6) linkages, and
(b) (desoxy)ribonucleic acids, as well as their cleavage and degradation products.
Surprisingly it was found that mixtures of &bgr;-(1,3) glucans, which are substantially free from &bgr;-(1,6) linkages, together with ribo- or desoxy ribonucleic acids show a synergistic improved absorption spectrum, which is especially well suited for protection of (desoxy)ribonucleic acids of the body. This effect, which has not been found in mixtures of nucleic acids with glucans, which still show appreciable fractions of (1,6) branches, can advantageously be increased by using as further components common UV light protection factors or antioxydants, which may be organic filters or anorganic pigments. The preparations according to the invention are further distinguished by the fact that they moderate skin irritations and injuries and stimulate the water and metabolic procedures in the skin, so that for example also the wrinkling because of desiccating due to heavy radiation from the sun at the same time is counteracted. As to the hair the film formation is supported.
Water Soluble &bgr;-(1,3) Glucans
The term glucans is intended to mean homopolysaccharides based on glucose. Depending on sterical linking there is a difference between &bgr;-(1,3), &bgr;-(1,4) and &bgr;-(1,6) glucans. &bgr;-(1,3) Glucans normally show a helical structure, whereas glucans with a (1,4) linkage generally have a linear structure. The &bgr;-glucans of the invention have a (1,3) structure, i.e. they are substantillay free from undesired (1,6) linkages. Preferably such &bgr;-(1,3) glucans are used where the side chains exclusively show (1,3) linkages. Especially the agents contain glucans which are obtained on the basis of yeast from the family Sacchaomyces, especially
Saccharomyces cerevisiae.
Glucans of this type are available in technical amounts according to known methods. The International patent application WO 95/30022 (Biotec-Mackzymal) describes e.g. a method for producing such substances, wherein glucans with &bgr;-(1,3) and &bgr;-(1,6) linkages are brought in contact with &bgr;-(1,6) glucanases in such a way, that practically all &bgr;-(1,6) linkages are loosened. Preferably used for the manufacture of these glucans are glucanases based on
Trichodermia harzianum.
As to the manufacture and availability of the glucans contained in these agents, reference is made to the above cited publication.
(Desoxy)ribonucleic Acids
As (desoxy)ribonucleic acids (DNA or RNA) is to be understood high molecular, thread-like polynucleotides, which are derived from 2′-desoxy-&bgr;-D-ribonucleosides or D-ribonucleosides, which again are built up of equivalent amounts of a nucleobase and pentose 2-desoxy-D-ribofuranose or D-ribofuranose. As nucleobases the DNA's or RNA's may contain the purine derivatives adenine and guanine as well as the pyrimidines cytosine and thymine or uracil. In the nucleic acids the nucleobases are N-glycosidic with the carbon atom 1 of the ribose, whereby in each individual case adenosine, guanosine, cytidine and thimidine developes. In the acids a phosphate group is connecting the 5′-hydroxy groups of the nucleosides with der 3′-OH group of the in each case following through a phosphodiester bridge under formation of single string DNA or RNA. Because of the high ratio of length to diameter DNA, respectively RNA, molecules tend to a string break already by mechanical strain, such as during the extraction. Therefore the molecular weight of the nucleic acids may be from 10
3
to 10
9
Daltons. For the purpose of the invention concentrated DNA o
Ansmann Achim
Engstad Rolf E.
Fabry Bernd
Gassenmeier Thomas Otto
Griesbach Ute
Barts Samuel
Biotec ASA
Henry Michael C.
Ladas & Parry
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