Coryneform expression and secretion system

Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Recombinant dna technique included in method of making a...

Patent

Rate now

  [ 0.00 ] – not rated yet Voters 0   Comments 0

Details

4351723, 4352523, 43525232, 435320, 935 29, 935 48, 935 72, C12P 2100, C12N 1500, C12N 120

Patent

active

049651978

ABSTRACT:
A coryneform expression and secretion system for homologous and heterologous genes consisting of the host, nucleotide sequences encoding a protein of interest, signals for expression and, optionally, targeting signals which direct membrane anchoring and for secretion and processing of the expressed protein. Regulatory signals may be utilized to control the rate and extent of expression and secretion. The system may further include compounds such as ionophores for altering the membrane transport of the host. The host itself may be mutated to alter transport, for example, by decreasing the mycolic acid content of Corynebacteria species.
The preferred host is a Corynebacterium although other coryneforms deficient in extracellular protease production may also be used. C. glutamicum is used as a model organism for the secretion system. This Gram positive, non-pathogenic bacteria can efficiently utilize heterologous expression and secretion signals originating from a variety of both Gram negative and Gram positive bacteria to provide the basis for the overproduction and secretion of cloned gene products in a given Corynebacterium host, as demonstrated by the expression and secretion by C. glutamicum of a lipase encoded by a gene from S. hyicus and a thermonuclease encoded by a gene from S. aureus.

REFERENCES:
patent: 4601983 (1986-07-01), Nakamori et al.
patent: 4649119 (1987-03-01), Sinskey et al.
patent: 4758512 (1988-07-01), Goldberg et al.
patent: 4778762 (1988-10-01), Miwa et al.
patent: 4830962 (1989-05-01), Gelfand et al.
Fahnestock et al., Applied and Environmental Microbiology 53: 379-384, 1987 (Feb.).
Follettie et al., J. Bacteriology 167: 695-702, 1986 (Aug.).
Yoshihama et al., J. Bacteriology 162: 591-597, 1985.

LandOfFree

Say what you really think

Search LandOfFree.com for the USA inventors and patents. Rate them and share your experience with other people.

Rating

Coryneform expression and secretion system does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Coryneform expression and secretion system, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Coryneform expression and secretion system will most certainly appreciate the feedback.

Rate now

     

Profile ID: LFUS-PAI-O-766404

  Search
All data on this website is collected from public sources. Our data reflects the most accurate information available at the time of publication.