Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing oxygen-containing organic compound
Reexamination Certificate
2008-05-06
2008-05-06
Achutamurthy, Ponnathapu (Department: 1652)
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Preparing oxygen-containing organic compound
C435S252300, C435S069100, C435S252320, C536S023200
Reexamination Certificate
active
10566129
ABSTRACT:
The present invention provides an aerobic coryneform bacterium transformant in which a lactate dehydrogenase gene is disrupted, and a pyruvate carboxylase gene is recombined so as to be highly expressed by a genetic engineering method. The aerobic coryneform bacterium transformant of the present invention can produce dicarboxylic acids from saccharides at a high production rate.
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Murakami et al., “Development of a New Bioprocess Invovling Cornye-form Bacteria-Analysis of the Pathway of Anaerobic Metabolism using High Gene Expression Strains”, The Meeting Summaries and Lecture Abstracts of the 2003 Annual Meeting of JSBBA (The Japan Society for Bioscience, Biotechnology, and Agrochemistry) held in Tokyo, Japan, Abstract 2A11p07, published Mar. 5, 2003. (with English translation).
G. N. Vemuri et al., “Effects of Growth Mode and Pyruvate Carboxylase on Succinic Acid Production by Metabolically Engineered Strains ofEscherichia coli”, Applied and Environmental Microbiology, vol. 68, No. 4, pp. 1715-1727, Apr. 2002.
R. R. Gokarn et al., “The Physiological Effects and Metabolic Alterations Caused by the Expression ofRhizobium etliPyruvate Carboxylase inEscherichia coli”, Applied Microbiology Biotechnology, vol. 56, No. 1-2, pp. 188-195, Jul. 2001.
A. M. Sanchez et al., “Efficient Succinic Acid Produciton from Glucose through Overexpression of Pyruvate Carboxylase in anEscherichia coliAlcohol Dehydrogenase and Lactate dehydrogenase Mutant”, Biotechnol. Prog., vol. 21, pp. 358-365, Mar. 2005.
Ikenaga Yuko
Inui Masayuki
Murakami Shikiko
Nakata Kaori
Okino Shohei
Achutamurthy Ponnathapu
Meah Md Younus
Research Institute of Innovative Technology for the Earth
Wenderoth, Lind and Ponack, L.L.P.
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