Coryneform bacterium transformant and process for producing...

Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing oxygen-containing organic compound

Reexamination Certificate

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C435S252300, C435S069100, C435S252320, C536S023200

Reexamination Certificate

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07368268

ABSTRACT:
The present invention provides an aerobic coryneform bacterium transformant in which a lactate dehydrogenase gene is disrupted, and a pyruvate carboxylase gene is recombined so as to be highly expressed by a genetic engineering method. The aerobic coryneform bacterium transformant of the present invention can produce dicarboxylic acids from saccharides at a high production rate.

REFERENCES:
patent: 6852516 (2005-02-01), Hibino et al.
patent: 11-196888 (1999-07-01), None
patent: 11-206385 (1999-08-01), None
patent: 99/53035 (1999-10-01), None
Murakami et al., “Development of a New Bioprocess Invovling Cornye-form Bacteria-Analysis of the Pathway of Anaerobic Metabolism using High Gene Expression Strains”, The Meeting Summaries and Lecture Abstracts of the 2003 Annual Meeting of JSBBA (The Japan Society for Bioscience, Biotechnology, and Agrochemistry) held in Tokyo, Japan, Abstract 2A11p07, published Mar. 5, 2003. (with English translation).
G. N. Vemuri et al., “Effects of Growth Mode and Pyruvate Carboxylase on Succinic Acid Production by Metabolically Engineered Strains ofEscherichia coli”, Applied and Environmental Microbiology, vol. 68, No. 4, pp. 1715-1727, Apr. 2002.
R. R. Gokarn et al., “The Physiological Effects and Metabolic Alterations Caused by the Expression ofRhizobium etliPyruvate Carboxylase inEscherichia coli”, Applied Microbiology Biotechnology, vol. 56, No. 1-2, pp. 188-195, Jul. 2001.
A. M. Sanchez et al., “Efficient Succinic Acid Produciton from Glucose through Overexpression of Pyruvate Carboxylase in anEscherichia coliAlcohol Dehydrogenase and Lactate dehydrogenase Mutant”, Biotechnol. Prog., vol. 21, pp. 358-365, Mar. 2005.

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