Coryneform bacteria DNA fragment containing a gene responsible f

Details Coryneform bacteria DNA fragment containing a gene responsible f Coryneform bacteria DNA fragment containing a gene responsible f

1994-05-18

1997-01-28

LeGuyader, John L.

Chemistry: molecular biology and microbiology

Micro-organism, per se ; compositions thereof; proces of...

Bacteria or actinomycetales; media therefor

4351721, 4351723, 4353201, 435840, 435843, 536 231, C07H 2104, C12N 1500, C12N 1574, C12N 1577

Patent

active

055977278

ABSTRACT:
The present invention relates to a DNA fragment which contains a gene responsible for the function of autonomous replication of plasmid in a Coryneform bacterium, said gene being obtained from plasmid pBY503 held in Brevibacterium stationis, and in which at least one point mutation capable of increasing the copy number of plasmid exists on said gene region. By using a Coryneform transformed with the vector constructed using said DNA fragment and an industrially useful gene such as aspartase gene or tryptophan synthase gene, production of the useful product occurs with higher efficiency than conventional methods.

REFERENCES:
patent: 4703012 (1987-10-01), Boros et al.
patent: 4874698 (1989-10-01), Ozaki et al.
patent: 4946781 (1990-08-01), Nakamori et al.
patent: 5185262 (1993-02-01), Kohama et al.

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