Corynebacterium glutamicum variety producing L-arginine and...

Details Corynebacterium glutamicum variety producing L-arginine and... Corynebacterium glutamicum variety producing L-arginine and...

2008-01-11

2011-11-01

Saidha, Tekchand (Department: 1652)

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing alpha or beta amino acid or substituted amino acid...

C435S252300, C435S252320

Reexamination Certificate

active

08048648

ABSTRACT:
The present invention relates to an L-arginine producing mutant strain, and a method for fabricating the same. In particular, the present invention relates to a polynucleotide comprising an argD2 gene (Ncgl2355) that is a putative gene of acetylornithine aminotransferase involved in arginine biosynthesis ofCorynebacterium glutamicum, a polypeptide encoded by the polynucleotide, a recombinant vector comprising the polynucleotide, a transformant capable of producing L-arginine in a high yield, which is prepared by introducing the recombinant vector into an L-arginine producing host microorganism to overexpress the argD2 gene, and a method for producing L-arginine by culturing the transformant. The transformant of the present invention overexpresses the argD2 gene to produce L-arginine in a high yield, thereby being used in medicinal and pharmaceutical industries.

REFERENCES:
patent: WO 01/00843 (2001-01-01), None
patent: WO 01/66573 (2001-09-01), None
McHardy et al. ( J. Biotechonol 2003, 104, pp. 229-240).
Sousa et al. Microbiology 148(Pt5):1291-1303, 2002.
European Search Report dated Dec. 11, 2009 re EP Appln. No. EP 08 70 4743.

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