Corynebacterium glutamicum strain with enhanced secretion...

Chemistry: molecular biology and microbiology – Micro-organism – per se ; compositions thereof; proces of... – Bacteria or actinomycetales; media therefor

Reexamination Certificate

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C435S069100, C435S252300, C435S320100, C530S350000, C536S023100

Reexamination Certificate

active

06982162

ABSTRACT:
The present invention refers to aCorynebacterium glutamicumbacterial strain which new isolated and identified secD and/or secF gene(s) is/are genetically modified in a way that secretion of the bacterial strain is enhanced, the protein- and polynucleotide sequences of these genes, a plasmid containing at least one of these genes and the use of such a bacterial strain for production of a desired substance or in a reporter system.

REFERENCES:
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patent: WO 01 00 804 (2001-01-01), None
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Attwood et al. Which craft is best in bioinformatics? Comput. Chem. 2001, vol. 25(4), pp. 329-339.
Ponting, C.P. Issues in predicting protein function from sequence. Brief. Bioinform. Mar. 2001, vol. 2(1), pp. 19-29.
Smith. Accession U00011. Mar. 1, 1994. (US-09-852-053-3 X U00011).
Smith. Accession U00011. Mar. 1, 1994. (US-09-852-053-4 X U00011).
Salim et al., “Heterologous expression of theMycobacterium tuberculosisgene encoding antigen 85A inCorynebacterium glutamicum”, Applied and Environmental Microbiology, vol. 63, No. 11, 1997, p. 4392-4400.
Billman-Jacobe et al., “Expression and Secretion of heterologous proteases byCorynebacterium glutamicum”, Applied and Evironmental Microbiology, vol. 61, No. 4, 1995, p. 1610-1613.
Pogliano et al., “SecD and SecF facilitate protein export inEscherichia coli”, Embo Journal, vol. 13, No. 3, 1994, p. 554-561.
Database EMBL Acc No. Z77724, 1996 XP002154142.

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