Correction method and measurement device for anti-stokes...

Details Correction method and measurement device for anti-stokes... Correction method and measurement device for anti-stokes...

2006-05-24

2010-11-02

Evans, F. L (Department: 2877)

Optics: measuring and testing

By dispersed light spectroscopy

With sample excitation

C356S307000, C356S318000, C356S417000

Reexamination Certificate

active

07826052

ABSTRACT:
A method to correct measurement error in a resonance energy-transfer assay, including exciting anti-Stokes photoluminescent donors with at least one wavelength of light which is greater than an emission wavelength of acceptor molecules; measuring emission at the acceptor's emission wavelength and which differs from the donor's emission wavelength in at least two different time windows; a first time window within the time window defined by the excitation light pulse and a second non-overlapping time window which follows the first time window; and correcting the emission signal, which includes signals originating from non-radiative and radiative energy transfer, within the first time window by estimating the ratio of the signals from non-radiative and radiative energy transfer or the signal originating from radiative energy transfer using at least one emission signal measured in the second time window.

REFERENCES:
patent: 2004/0076948 (2004-04-01), Petterson
patent: 2004/0076979 (2004-04-01), Belik et al.
patent: WO 98/15830 (1998-04-01), None
patent: WO 99/12018 (1999-03-01), None
patent: WO 2005/033709 (2005-04-01), None

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