Correction for illumination non-uniformity during the...

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid

Reexamination Certificate

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C435S091100, C435S283100, C435S287200, C536S023100, C536S025300, C422S082050, C359S212100

Reexamination Certificate

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07422851

ABSTRACT:
The present invention provides an apparatus and a method for the synthesis of arrays of oligomers that have tight illumination intensity requirement. The apparatus and the method contains a mechanism to correct for illumination nonuniformity during the oligomer array synthesis process. To correct for illumination nonuniformity, the illumination intensity of different oligomer synthesis positions across an area in which oligomers are synthesized are determined first. Then, the difference in illumination intensity among the positions are evaluated mathematically. Next, any nonuniformity in illumination intensity is corrected by either reducing the intensity of the light for the brighter positions before the light reaches the illumination area or reducing the illumination time of the brighter positions during one protection group deprotection period.

REFERENCES:
patent: 5843655 (1998-12-01), McGall
patent: 5870176 (1999-02-01), Sweatt et al.
patent: 6262795 (2001-07-01), Baker et al.
patent: 6271957 (2001-08-01), Quate et al.
patent: 6344641 (2002-02-01), Blalock et al.
patent: 2003/0002040 (2003-01-01), MacAulay et al.
U.S. Appl. No. 60/296,635.

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