Chemistry: molecular biology and microbiology – Process of mutation – cell fusion – or genetic modification – Fusion of cells
Reexamination Certificate
2005-08-16
2005-08-16
Ketter, James (Department: 1636)
Chemistry: molecular biology and microbiology
Process of mutation, cell fusion, or genetic modification
Fusion of cells
C435S461000, C435S470000, C435S285200
Reexamination Certificate
active
06929949
ABSTRACT:
The present invention provides a method and apparatus adapted to facilitate the entry of a preselected molecule into the intracellular space of a cellular sample through the use of ions generated by a corona charge source. With the present method and apparatus, molecules are manipulated within cells and in the extracellular space surrounding the cells. Manipulation enhances the permeability of cell barriers to allow the subsequent introduction of molecules of interest into the interior of a cell.
REFERENCES:
patent: 4413897 (1983-11-01), Kohyama
patent: 4656356 (1987-04-01), Yoda et al.
patent: 5869326 (1999-02-01), Hofmann
patent: 5983131 (1999-11-01), Weaver et al.
Gilbert Richard
Heller Richard
Hoff Drew
Jaroszeski Mark J.
Ketter James
Sauter Molly L.
Smith & Hopen , P.A.
University of South Florida
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