Copper tolerant yeast and pectinases produced by the yeast

Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Hydrolase

Reexamination Certificate

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C435S004000, C435S006120, C435S041000, C435S069100, C435S183000, C435S200000, C435S252300, C435S254100, C435S254200, C435S243000, C435S262000, C435S275000, C536S023200

Reexamination Certificate

active

10252093

ABSTRACT:
The object of the present invention is to provide a yeast which is tolerant to copper and which can incorporate copper at a high concentration, and also a method of removing or recovering copper from extracellular solution. The present invention is copper-tolerant yeast and the pectinases produced by the yeast. Particularly, the present invention is copper-tolerant yeastCryptococcussp. N6 strain isolated from deep-sea sediments and the pectinases produced by the yeast.

REFERENCES:
Karel Grohmann et al.,Fermentation of Orange Peel Hydrolysates by Ethanologenic Escherichia coli, Appplied Biochemistry and Biotechnology, vol. 57/58, 1996, pp. 383-388.
Federico Federichi,Production, Purification and Partial Chracterization of an Endo-polygalacturonase from Cryplococcus albidus var, albidus, Antonie van Leeuwenhoek, vol. 51 No. 2, (1985), pp. 139-150.
Hirotsugu Imahara et al.,Effect of Copper on Growth of Yeast, Agric. Biol. Chem., 42 (6), 1173-1179, 1978.
K. C. Gross, Hortscience, vol. 17, No. 6, pp. 933-934, “A Rapid and Sensitive Spectrophotometric Method for Assaying Polygalacturonase Using 2-Cyanoacetamide”, 1982.
F. Federici, Boll. Soc. Ital. Biol. Sper., vol. 59, pp. 1509-1512, “Preliminary Study of the Pectolytic Activity ofCryptococcus albidusVar. Albidus”, 1983.
A. M. McKay, FEMS Microbiology Letters 56, pp. 355-358, “A Plate Assay Method for the Detection on Fungal Polygalacturonase Secrection”, 1988.
F. Abe, et al. JAMSTEC J. Deep Sea Res., vol. 14, pp. 573-580, “Characterization of Copper Tolerant Deep-Sea YeastCryptococcusSP. N6 Isolated From the Japan Trench”, 1998 (with partial English translation).
T. Miura, et al., International Congress on Extremophiles '98, p. 334, “Isolated and Characterization of a Copper Tolerant YeastCryptococcusSP. From the Japan Trench”, Jan. 18-22, 1998.
Program of the Annual Meeting 1999 of The Japan Society for Bioscience, Biotechnology, and Agrochemistry, vol. 73, No. 3, pp. 17, 54 and 34, Mar. 30-Apr. 1, 1999 (with English translation).
T. Miura, et al., Proceedings of 4thSymposium on Bio Nano Electronics, p. 74, “Purification and Characterization of Extracellular Endopolygalacturonases of the Copper Tolerant YeastCryptococcusSP. N6 Isolated From the Japan Trench”, Nov. 15, 1999.

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