Copper oxidation protein assay

Details Copper oxidation protein assay Copper oxidation protein assay

1991-10-12

1992-12-22

Housel, James C.

Chemistry: analytical and immunological testing

Peptide, protein or amino acid

436164, 436805, 422 56, 558162, 558209, G01N 3348

Patent

active

051734311

ABSTRACT:
A procedure for detecting proteins in fluids (particularly body fluids) which involves contacting such fluid with a composition comprising copper in a form capable of forming a copper/protein complex, a peroxide and a redox indicator which provides a detectable response when oxidized, together with an ionizable phosphate containing compound to prevent interference with the redox indicator from substances present in the fluid.

REFERENCES:
Yamamoto et al., Int. J. Biol. Macromol., 1980 vol. 2, Aug. pp. 263-265 "Catalytic actions of synthetic polypeptides: 1. Decomposition of hydrogen peroxide by the poly(L-glutamic acid)-copper (II) complex".
Mack et al., J. Am. Chem. Soc. 1988 vol. 110, pp. 7572-7574 Design and Chemical Synthesis of a Sequence-Specific DNA-Cleaving Protein.

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